Aflatoxin B1 ELISA Kit

الوصف

1. Principle
This test kit is based on the competitive enzyme immunoassay for the qualitative,quantitative detection of Aflatoxin B1 in the Corn, rice, wheat, beans, peanuts, peanut butter, cooking oil.The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxin B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Aflatoxin B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Aflatoxin B1 concentration in the sample. This value is compared to the standard curve and the Aflatoxin B1 concentration is subsequently obtained.

2. Technical specifications
Sensitivity : 0.1ppb
Detection limit:
 Peanuts, peanut butter; corn, wheat and other grains; cookies, honey 2ppb
 Feed 2.4ppb/1.*5ppb
 Cooking oil 1.5ppb
 Soy sauce, vinegar, wine 1ppb
 Dry miso, hot pot materials 1ppb
Recovery rate:
 Peanuts, peanut butter; corn, wheat and other grains *0±*5%
 Cookies *0±*5%
 Honey cake *5±*5%
 Feed *5±*5%
 Cooking oil *0±*5%
 Soy sauce, *5±*5%
 Vinegar *0±*5%
 wine *0±*5%
 Dry miso *0±*0%
 Hot pot materials *5±*0%
 
Cross-reaction rate:
 Aflatoxin B1 **0%
 Precision:Intra-assay CV<*0%;Inter-assay CV<*0%