الوصف

INTENDED USE

TheAvian Influenza Virus Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Avian Influenza Virusantigen(AIV Ag) in avian serum, secretions or spleen.

Assay Time: 5**0 minutes

Specimen: serum, secretions or spleen

PRINCIPLE

TheAvian Influenza Virus Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated samplewas applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is AIV antigen in the specimen, a visible Tline will appears. The C line should always appear after a sample is applied,which indicates a valid result. By this means, the device can accurately indicate the presence ofAIVvirus antigen in thespecimen.

REAGENTS AND MATERIALS

- *0Test devices

- Disposable droppers

- 1 bottle of assay buffer  

- *0Cotton swabs

- 1 Products Manual

STORAGE AND STABILITY

The kit can be stored at room temperature (***0°C). The test kit is stable through the expiration date (*8 months) marked on the package label.DO NOT FREEZE. Do not store the test kit in direct sunlight.

TEST PROCEDURE

- Dip the swab stick from the birds eyes, trachea, cloaca to collect the secretion. Please make the swab wet sufficiently.

- Insert the wet swab into the provided assay buffer tube. Agitates the swab to assure good sample extraction.

- If blood sample, it is suggested to collect 0.**1.0mL of blood and do a centrifugation at ***0 rpm. Use the serum directly in the assay.

- Take out the cassette from the foil pouch and place it horizontally.

- Suck the sample extraction liquid or serum and place 3 drops into the assay sample hole S.

- Interpret the result in *0 minutes. Result after *0 minutes is considered as invalid.

INTERPRETATION OF RESULTS

Positive: The presence of both Cline and zone Tline, no matter Tline is clear or vague.

Negative:Only clear C band appear. No T band.

Invalid:No colored band appears in C zone. No matter if T band appears.

PRECAUTIONS

- All reagents must be at room temperature before running the assay.

- Do not remove test cassette from its pouch until immediately before use.

- Do not use the test beyond its expiration date. 

- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.

LIMITATION

Avian Influenza Virus Antigen Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian.It is suggested to apply a further confirmative method such as RT-PCR when positive result was observed.