الوصف

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The Centri-Sep gel will provide excellent recovery of DNA fragments with sizes greater than *6 base pairs or **-mer, while removing > *8 % of salts, NTP's and other unwanted low- molecular- weight impurities.

The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low- molecular- weight components, but also exchanged into the buffer of your choice.

 

• Removal of excess DyeDeoxy™ terminators
• Removal of excess BigDye™ terminators
• Removal of more than *8% of salts and unwanted low-molecular-weight impurities
• Purification of fluorescent reaction mixtures
• Removal of free and labeled dNTP's from DNA or RNA, as inon:

1. nick translation
2. end-labeling reactions
3. polymerization reaction

• Removal of traces of phenol or exchange of buffer salts
• Purification and desalting of proteins
• Removal of excess nucleotides and small primers in PCR reactions
• Removal of radioactive precursors
• Desalting and buffer exchange
• These columns are far superior – easy to use, quick protocol, non-toxicity – than phenol/chloroform extraction or ethanol precipitation.

• Processed faster than ultrafiltration columns
• Rapid and efficient separations
• Buffer is not preselected
• Convenient *****0 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format

• Reliable and consistent
• Time-saving and convenient
• Enhanced stability
• Extended shelf-life
• No special equipment
• Extremely versatile.