Human von Willebrand Factor antigen, vWF:Ag ELISA Kit

الوصف

Intended use This immunoassay kit allows for the in vitro quantitative determination of human vWF:Ag concentrations in serum, plasma and other biological fluids. Test principle The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF:Ag. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF:Ag. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain vWF:Ag, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of **0 nm 2 nm. The concentration of vWF:Ag in the samples is then determined by comparing the O.D. of the samples to the standard curve.