ISO13485 certification AMH elisa test kit

الوصف

AMH elisa test kit


Intended use
Immunoassay for the in vitro quantitative determination of Anti-Mullerian Hormone
in human serum.
Summary
Anti-Mllerian hormone (AMH), also known as Mllerian-inhibiting hormone (MIH),
is a glycoprotein hormone structurally related to inhibin and activin from the
transforming growth factor beta superfamily, whose key roles are in growth
differentiation and folliculogenesis.(1) In humans, the gene for AMH is AMH, on
chromosome *9p*3.3,(2) while the gene AMHR2 codes for its receptor on
chromosome *2.(3)
AMH can be used for selection of females in multi-ovulatory embryo transfer
programs by predicting the number of antral follicles developed to ovulation.(4)
AMH can also be used as a marker for ovarian dysfunction, such as in women
with polycystic ovary syndrome (PCOS).
Test principle
Sandwich principle. Total duration of assay: *0 minutes.
• Sample, Anti-AMH coated microwells and enzyme labeled Anti-AMH are
combined.
• During the incubation, AMH presents in the sample is allowed to react
simultaneously with the two antibodies, resulting in the AMH molecules being
sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the AMH within
the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a
chromogenic reaction. The resulting chromogenic reaction is measured as
absorbance.
• The absorbance is proportional to the amount of AMH in the sample.