Lyndizyme Cyclization Thermostability Phytase

الوصف

The new technology of directed molecular evolution of enzyme has become a hotspot of research in the world to improve the enzyme efficiency. In this field, leading subject is the cyclization of protein, which not only is a basic research for developing new generation of technology, but also bears a significant role in promoting the growth of enzyme-related industry. Habio cyclic thermostability phytase is a new kind of cyclic protein based on the protein self-splicing. The two ends of phytase were linked with the N-terminus and C-terminus of protein “intron” intein respectively to construct the expression vector of fusion gene. The fusion protein was induced to overproduction and cyclized by self-splicing of intein. Comparing with linear protein, the conformation changeability of cyclic protein is reduced and the enzyme is in an active state. As results, the stability and activity of cyclic phytase are much better. With no need of coating, Habio cyclization thermo stability phytase can be released fast in gastro-intestinal tract of animals, biology efficiency is enhanced distinctly in the same time. The N-terminus and C-terminus of cyclic protein was linked without any change of three-dimensional conformation (as following figure), thus kept the protein in active state. Superior quality 1、Higher thermostablity and higher remaining rate after feed granulating compared with other similar products. 2、there is no change on the three-dimensional conformation of Habio cyclic thermostability phytase; Protein will not lose activity, having more stable performance. Higher biological efficiency could be achieved by combining and decomposing substrates more effectively and no need to release in animals’ the gastrointestinal tract because of no more coating. 3、Habio Cyclization Thermostability Phytase can not only improve the stability, but also improve the biological value of phytase. Technical service Offering phytase usage formula, enzyme activity test method and related product technical service.