الوصف

[Product Name]
One-step Detection Kit for Hepatitis B virus (QPCR-Probe)
[Packing Specification]*4rxns/kit
[Intended Use]
This kit is used for the qualitative detection of Hepatitis B Virus (HBV) in whole blood and serum samples. It offers auxiliary means for the diagnosis of the HBV infected patients without nucleic acid extraction and purification. Test results should be combined with clinical diagnosis.
[Detection Principle]
This kit adopts PCR method combined with fluorescence probe in vitro amplification technology. In this method, the HBV probe contains a fluorescent reporter dye FAM at the 5'end of the probe and a quencher dye BHQ at the 3'end of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye suppresses the reporter fluorescence. Probe cleavage during the PCR reaction spatially separates the reporter dye from the quencher dye,
thereby allowing detection of the reporter dye fluorescence. The fragments of reporter dye are displaced from the target, resulting in an increase in fluorescence. This step, which enables the fluorescence signal accumulation and PCR products formed synchronous, thus to achieve qualitative detection the HBV in the infective patient in serum samples, which provide auxiliarymeans for the HBV infection in the treatment of the patient. 
 [Quality control]
If the Ct value of positive control sample≤*5, and the Ct of the negative control is 0 or>*8, this test is valid,or the test is invalid.
[Results interpretation]
a.If the Ct of sample ≤*8, report positive for the HBV;
b.If the Ct of sample *0>ct>*8, please test again. If the duplicate detection Ct of sample>*8, negative control Ct value is none, reported positive for the HBV. If the duplicate detection Ct of sample is 0 or none, reported negative for the HBV; c. If the Ct of sample is no value or>*8, report negative for the HBV.