سعر فوب
أحصل على آخر سعر|
1 Piece Minimum Order
بلد:
China
نموذج رقم:
P1021-P1024
سعر فوب:
الموقع:
China
سعر الحد الأدنى للطلب:
-
الحد الأدني للطلب:
1 Piece
تفاصيل التغليف:
small package of bulk, distribute of OEM, shipped with ice bag
موعد التسليم:
1-2weeks
القدرة على التوريد:
100 Piece per Week
نوع الدفع:
T/T, L/C, Western Union
مجموعة المنتج :
الشخص الذي يمكن الاتصال به Ms. vivian
89 yanlingroad, Guangzhou, Guangdong
Description
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is *0 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 0.2~0.4kb/min(*0~*5°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of *0 nmole of dNTPs into an acid-insoluble form in *0 minutes at *0°C using hering sperm DNA as substrate.
Storage Buffer
*0mM TrisCl ( pH8.0), **0mM KCl, 3mM MgCl2 1mM DTT,0.1% NP**0 ,0.1% Tween*0, 0.2mg/ml BSA, *0% (v/v) glycerol
*0X Pfu Buffer with MgSO4
**0mM Tris-HCl(pH8.8,*5°C), **0mM KCl, **0mM (NH4)2SO4, *0mM MgSO4, 1.0% Triton®X***0, 1mg/ml BSA
.Applications
• High-fidelity PCR and primer-extension reactions.
• High fidelity PCR for cloning into blunt-ended vectors.
• Site-directed mutagenesis.
بلد: | China |
نموذج رقم: | P1021-P1024 |
سعر فوب: | أحصل على آخر سعر |
الموقع: | China |
سعر الحد الأدنى للطلب: | - |
الحد الأدني للطلب: | 1 Piece |
تفاصيل التغليف: | small package of bulk, distribute of OEM, shipped with ice bag |
موعد التسليم: | 1-2weeks |
القدرة على التوريد: | 100 Piece per Week |
نوع الدفع: | T/T, L/C, Western Union |
مجموعة المنتج : | PCR Reagent |