الوصف
Protein A medium / rProtein A Sepharose 4 Fast Flow is a well
established antibody affinity medium, designed for
the purification of monoclonal and polyclonal antibodies at
both laboratory and process scale.
· High dynamic binding capacity due to oriented, single-point
attachment of protein A
· Well-established Protein A medium used in many approved MAb
processes
· No mammalian components involved during the manufacturing
process
rProtein A Sepharose 4 Fast Flow is based on the well
established Sepharose Fast Flow
platform and is used in many approved monoclonal antibody
(MAb) processes. It is composed of
recombinant protein A coupled to the cross-linked 4% agarose
base matrix. The recombinant
protein A is produced in E. coli and has been specially
engineered to favour an oriented coupling
giving a matrix with enhanced binding capacity. The epoxy
based coupling ensures low ligand
leakage. The specificity of the recombinant protein A for the
Fc region of IgG is similar to native
protein A, giving a high purification factor in a single step.
The high capacity, low ligand leakage
and a well established base matrix makes rProtein A Sepharose
4 Fast Flow suitable for
purification of monoclonal antibodies at both laboratory and
process scale.
rProtein A Sepharose 4 Fast Flow is available in a range of
different bulk pack sizes and
convenient pre-packed formats for easy scale-up and process
development.
As member of the BioProcess media range, rProtein A Sepharose
4 Fast Flow meets industrial
demands with security of supply and comprehensive technical
and regulatory support
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