الوصف
Description
　
　Biologically active BMP*2 is a homodimer, linked by a
single disulfide bond. The monomers have three intrachain
disulfide bridges, forming a so-called cystine knot. In vivo, the
monomer is synthesized as a large precursor, which after
dimerization, is prote-olytically cleaved releasing the smaller
C-terminal fragment forming the mature dimer
.
Our product
produced in genetically engineered Eschericha coli is a
non-active aggregated form. After refolding and purification, the
recombinant human bone morphogenetic protein*2 is active and with
high homogeneity.
Source
:Escherichia
Coli.
Physical
Appearance
:Sterile
Filtered White lyophilized (freeze-dried)
powder.
Formulation
:Lyophilized
from a concentrated (1mg/ml) solution in water containing *0mM
Sodium Acetic buffer, pH4.0.
Solubility
:It
is recommended to reconstitute the lyophilized BMP*2 in distilled
H2O not less than **0ug/ml, which can then be further diluted to
other aqueous solutions.
Stability
:Lyophilized
BMP*2 although stable at room temperature for 3 weeks, should be
stored desiccated below **8℃. Upon reconstitution BMP*2 should
be stored at 4℃ between **7 days and for future use below
**8℃ . For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
　　Please avoid freeze-thaw
cycles.
Biological
Activity
Recombinant human
BMP*2 is fully biologically active when compared to standards.
When implanted in the mouse thighbone muscle and grow for *4
days, Muscle tissue was harvested and examined biochemically for
calcium uptake. Our unit defined as 1 microgram calcium absorbed.
The specific activity is more than ***0
Units/mg
| بلد: |
China |
| نموذج رقم: |
006
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