يبدو أنك لست عضوًا في TradeKey.com بعد. اشترك الآن للتواصل مع أكثر من 7 مليون مستورد ومصدر عالميًا.
انضم الآن ، مجانًا |
Book call on your favorite time

By Signing Up. I agree to TradeKey.com Terms of Use, Privacy Policy, IPR and receive emails related to our services

Amazon Services
عضو مجاني

Recombinant nuclease

China

Recombinant nucleae

1 ~ 5 / Milligram أحصل على آخر سعر

Zhejiang, PRC

1 per Milligram

1000 Unit

10000

ONE week

100000 Unit per Month

T/T, L/C

-

الاتصال الآن

مواصفات المنتج

Other Names : recombinant nuclease Type : Enzyme Preparations Brand Name : INDUMY CAS No. : - MF : - EINECS No. : - FEMA No. : -
colorless sotlution : colorless

الوصف

INDUMY nuclease is a recombinant endonuclease expressed in E.coli and purified to homogeneity. This enzyme attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length which is below the hybridization limit. NDUMY nuclease preparations possess exceptionally high specific activities and are supplied free from measurable protease activities and viral contaminants.

This enzyme is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.

• Viscosity reduction in protein extracts

• Sample preparation for 2D gel electrophoresis

• Removal of nucleic acid contaminants from recombinant protein preparations

This enzyme is designed with (His)6 tag and so it can be easily removed during the downstream procedures. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, The product is supplied as a 0.2 μm filtered solution in 50% glycerol.

Unit definition

One unit of INDUMY™ Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in

30 min, which corresponds to complete digestion of 37 μg of DNA. Standard reaction conditions

are 1 mg/ml sonicated DNA substrate in 50 mM Tris-HCl pH 8.0, 0.1 mg/ml BSA, 1 mM MgCl2,

incubated at 37°C; measured as perchloric acid-soluble digestion product.

Storage

INDUMYâ„¢ Nuclease is supplied in 50% glycerol containing 50 mM Tris-HCl pH 8.0,20 mM NaCl

and 2 mM MgCl2. The enzyme preparation is stable for 2 years when stored at –20°C.

DO NOT store at –70°C


 

اتصل بنا

الشخص الذي يمكن الاتصال به:Mr. Liu

الشركة:Hangzhou Biodoor Biotechnology Co,Ltd

عنوان:Xiangyuan Road 39#, Hangzhou, Zhejiang, China,310011

رقم الهاتف:

عرض رقم الهاتف

التليفون المحمول:

عرض رقم الجوال

رقم الفاكس:

عرض رقم الفاكس

إلى,

Mr. Liu < Hangzhou Biodoor Biotechnology Co,Ltd >
أريد أن أعرف:

لمزيد من المنتجات من موردين آخرين

glucono delta lactone glucono delta lactone
Alpha amylase Enzyme Cellulase Enzyme Protease Enzyme Alpha amylase Enzyme
High temperature alpha amylase High temperature alpha
Coenzyme Q10 CWS Coenzyme Q10 CWS
Beta-amylase Beta-amylase
DETERGENT ENZYME DETERGENT ENZYME
Pepsin Pepsin
beta-amylase beta-amylase
paper pulp enzyme paper pulp enzyme
Coenzyme Q10 Capsule Coenzyme Q10 Capsule
Supply enzyme-glucoamylase Supply enzyme-glucoamylase
Lipase Enzyme Lipase Enzyme
Thermostable alpha-amylase Thermostable alpha-amylase
Ayurjyothe Mosquito Coils Ayurjyothe Mosquito Coils
cellulase cellulase
Transglutaminase Transglutaminase