Infectious bronchitis virus (IBV)Antibody ELISA Kit

Description

INTENDED USE Enzyme immunoassays for the qualitative of IgG antibodies against IBV in Chicken serum or plasma Principles The IBV 2XR Test Kit is an enzyme immunoassay designed to detect the presence of antibody to IBV in chicken serum. A microtitration format has been configured by coating IBV antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to IBV forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-Chiken: horseradish peroxidase conjugate is added that binds to any Chiken IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, tetramethylbenzidine (TMB), are added into the wells. Stop the reaction by adding stop solution. Within *5 minutes READ the absorbance at **0nm using **0nm as the reference wavelength if available.