Nitrofuan (AOZ) ELISA Test Kit

Description

Nitrofural metabolite (SEM) ELISA Test Kit Nitrofural metabolite (SEM) ELISA test kit is mainly used to detect SEM and NP-SEM in the meat, chicken, fish, shrimp , honey, , etc. 1. Principle This test kit is based on the enzyme immunoassay and indirect competition for the detection of SEM in the sample. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with SEM concentration in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained. 2. Technical specifications Sensitivity : 0.*5ppb Detection limit: honey 0.3 ppbï&frac*4;› fish 0.4 ppbï&frac*4;› shrimp 0.4 ppbï&frac*4;› chicken 0.4 ppbï&frac*4;› liver(chicken) 0.4 ppbï&frac*4;› pork 0.4 ppbï&frac*4;› liver(pig) 0.4 ppbï&frac*4;› Recovery rate: honey *5±*5%; fish *0±*5%; shrimp *0 ±*5%; chicken *0 ±*5%; liver(chicken) *0 ±*5%; pork *0 ±*5%; liver(pig) *0 ±*5%;ï&frac*4;› Cross-reaction rate: SEM **0ï&frac*4;… AOZ <0.1ï&frac*4;… AMOZ <0.*1ï&frac*4;… AHD <0.*1ï&frac*4;… 3. Components 1. Micro-well strips: *2 strips with 8 removable wells each, coating with NP-SEM ovalbumin 2. 6 of standard solution (1 mL each): 0 ppb, 0.*5 ppb, 0.*5 ppb, 0.*5 ppb, 1.*5 ppb and 4.*5 ppb 3. *0 ×Enzyme conjugate (1.6 mL) 4. *0 ×concentrated antibody (0.8mL) 5. Substrate A solution (7mL), Substrate B solution (7mL) 6. Stop solution (7 mL) 7. *0× concentrated washing buffer (*0 mL) 8. 2× concentrated Sample diluent (*0 mL) 9. o-Nitrobenzaldehyde(C7H5NO3)( *5 mg) 4. Materials required but not provi