Description

Rapid Test Kit for Melamine 1. Principle This kit is based on competitive indirect immunochromatography technology, in which melamine in sample competes for the colloid gold labeled antibody with melamine coupling antigen captured on test line. The test result can be watched by naked eye. 2. Application This kit is intended for rapid test of melamine residue in milk powder 3. Detection limit Detection limit for milk sample: 1Î&frac*4;g/g 4. Kit components melamine test strip *************************0 pcs Pipette ****************************************0 pcs extraction solution **************************0ml Dilution buffer for milk powder sample*********5ml 5. Sample preparation *****-Dilute the dilution buffer with deionized water in the volume ratio of 1:4 . *****-Weigh 0.5±0.*5g milk powder into a *0ml pp tube, add 5ml extraction buffer, and then shake for 5min to mix completely. *****-Dilute the prepared solution with dilution buffer in the volume ratio of 1:1 (**0Î&frac*4;l milk powder solution : **0Î&frac*4;l dilution buffer), mix completely. 6. Assay procedure *****-Take the test strip from the package, and use for assay within 1 hour. *****- Place the test strip at a flat level, pipette the prepared sample solution, add 3 drops into the sample region. *****- Start the timer as the added solution flows, observe the result within **5min, determine the result according to part 7. Result observed over 6min is invalid. 7. Result interpretation Negative (-): Test Line and Control Line are both red, indicating that the melamine in sample is lower than the LOD of the kit. Positive (+): No color shows in Test line, indicating that the melamine in sample is higher than the LOD of the kit. Invalid: No color shows in Control Line, indicating the operation is incorrect or the test kit is out of date. In this case, please read the instruction again carefully, and do the assay again with new strips. HS Code: *******0 Trademark: GloryScience