Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit

Description

Catalog No.            
E-EL-H***4
Detection Range    
NA
Sensitivity             
NA
Specificity             
This kit recognizes natural and recombinant Human NOX5. No significant cross-reactivity or interference  between Human NOX5 and analogues was observed. Note：Limited by existing techniques, cross-reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human NOX5 and all the analogues.
                              
Repeatability         
Coefficient of variation were<*0%.</span>
 
Test principle         
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX5.Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human NOX5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of **0 nm ± 2 nm. The OD value is proportional to the concentration of Human NOX5. You can calculate the concentration of Human NOX5 in the samples by comparing the OD of the samples to the standard curve.