Aflatoxin B1 ELISA Kit

Description

Principle
Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereal grains and feed , etc.
Aflatoxin B1 ELISA Test Kit incorporates a fast, low-cost sample prep protocol with high recovery rates.

An antibody with a affinity for Aflatoxin B1 is coated onto microwells. Standard or sample is added to the appropiate well and if Aflatoxin B1 is present it will bind to the coated antibody.Subsequently, Aflatoxin B1 bound to horse-radish peroxidase (HRP) is added and binds to the antibody not already occupied by Aflatoxin B1 present in the sample or standard.After this incubation period,the contents of the wells are decanted,wahed and an HRP substrate is added which develops a blue color in the present of an enzyme.The resulting color intensity, has an inverse relationship with the target concentration in the sample. The levels of Aflatoxin B1 in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Aflatoxin B1 standards provided in the kit.

Detect Limit : 
             Grains************************2.5 ppb
              Feed***************************2.5 ppb
              Oil**************************2.5 ppb
              Food*************************1ppb

Specification: *6 tests /kit
Recovery rate:  *5 ±*5%