This kit is a colloidal gold immunochromatographic testing product
for the qualitative determination of IgG and IgM antibodies against
COVID**9 in human serum, plasma, whole blood, or fingertip blood.
Antibody-antigen sandwich one-step assay has been used in this
detection. For the test line (M line and G line), colloidal gold
conjugated COVID**9 antigen is used as a detector and coated on the
conjugate pad, and mouse anti-human IgM antibody and mouse
anti-human IgG antibody are used as the two captures and coated
separately on nitrocellulose membrane. For the quality control line
(C line), colloidal gold conjugated rabbit IgG is used as a
detector and coated on the conjugate pad and the goat anti-rabbit
IgG antibody is used as the capture and coated on nitrocellulose
membrane. When a sample is loaded on the conjugate pad, the IgM or
IgG antibody against the virus antigen in the sample specifically
binds to the virus antigen that is conjugate with gold, forms an
IgM or IgG antibody-antigen gold complex, then the complex migrates
on the nitrocellulose membrane and is captured by mouse anti-human
IgM or IgG antibody and immobilized on the nitrocellulose membrane,
and the red bands appear on the detection line (M line and G
line). The gold-labeled rabbit IgG is captured by a goat
anti-rabbit IgG antibody and forms a red quality control line (C
line) on the nitrocellulose membrane at the same time.
