Description

I. Product introduction
Recombinant trypsin: CAS: ********7; EC: 3.4.*1.4
Recombinant trypsin is a serine protease, which can specifically cleave the C-terminal peptide end of lysine and arginine.
Trypsin is widely used in various biotechnology processes. For example: proteolysis to produce polypeptides; cell separation of various tissues; proteolysis and sequencing; recombinant insulin production and so on.
 
II. Product characteristics
Source: Pichia pastoris
Character: white or similar white freeze-dried powder
Purity (SDS-PAGE): single main strip
Specific activity: ≥ ***0 USP units / mg Pro
Specificity: cleavage of the carboxy terminal peptide bond between arginine and lysine.
 
III. Product use
Production of recombinant insulin and analogs; cell culture and fermentation; enzymatic hydrolysis of proteins; cell separation of various tissues.
 
Iv. Recommend Usage
 It is recommended to use 1mM HCl solution to dissolve, The ration to aimed protein is 1:*0 to 1:***0, optimal ph 7.**9.0, optimal temperature ****5℃.
Note: The activity is inhibited by serine protease inhibitors such as PMSF, metal ion chelating agents such as EDTA, etc.; high concentration of inorganic salts will have certain effects on enzyme activity.
 
V. Storage stability
The lyophilized powder can be stored for more than 2 years at temperatures below *5 °C.
 
Vi. Product advantages
Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.
Stable quality: stable and continuous batch production can be guaranteed; T here is no difference between product batches and the quality is stable.
Lyophilized powder: easy to store and transport