الوصف

Catalog no.:                    SG*****5
Reactivity:                      Human
Methode Type:              Sandwich ELISA Detection             
Quantity:                        *6 tests
Sample type：               serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range                0.8ug/L -*0ug/L
The kit is for the quantitative level of Osteopontin  in the sample, adopt purified Human Osteopontin  to coat microtiter plate, make solid-phase antibody, then add Osteopontin  to wells, Combine Osteopontin  antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of **0 nm. The concentration of Osteopontin  in the samples is then determined by comparing the O.D. of the samples to the standard curve.