الوصف

The Sulfonamides (SAs*5) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamideHRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. Take coloration with TMB substrate. The samples A value is an inverse relationship with SAs residue content. Lastly, compared with the standard curve can be concluded the SAs*5 residues in sample.