Human Sclerostin (SOST) ELISA Kit

intended use

 

This immunoassay kit allows for the in vitro quantitative determination of Human Sclerostin (SOST) concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

sample preparation

 

1. DL Sci&Tech Development Co.Ltd is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.

 

2. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.

 

3.If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.

 

4. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.

 

5. Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.

 

6. Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.

 

7. Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

 

calculation of the result

 

Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve on log-log graph paper, with Human Sclerostin (SOST) concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.*0, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

 

Note

 

The Stop Solution suggested for use with this kit is an acid solution.Wear eye,hand,face and clothing protection when using this material.