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Products Introduction:
intended use
This immunoassay kit allows for the in vitro
quantitative determination of rat Insulin Like
Growth Factor Binding Protein 2 (IGFBP2)
concentrations in serum, Plasma, Urine, tissue
homogenates and Cell culture supernates and
Other biological fluids.
sample preparation
1. DL Sci&Tech Development Co.Ltd is only
responsible for the kit itself, but not for the
samples consumed during the assay. The user
should calculate the possible amount of the
samples used in the whole test. Please reserve
sufficient samples in advance.
2. Please predict the concentration before
assaying. If values for these are not within
the range of the standard curve, users must
determine the optimal sample dilutions for
their particular experiments.
3.If the samples are not indicated in the
manual, a preliminary experiment to determine
the validity of the kit is necessary.
4. Tissue or cell extraction samples prepared
by chemical lysis buffer may cause unexpected
ELISA results due to the impacts from certain
chemicals.
5. Due to the possibility of mismatching
between antigen from other origin and antibody
used in our kits (e.g., antibody targets
conformational epitope rather than linear
epitope), some native or recombinant proteins
from other manufacturers may not be recognized
by our products.
6. Influenced by the factors including cell
viability, cell number or sampling time,
samples from cell culture supernatant may not
be detected by the kit.
7. Fresh samples without long time storage is
recommended for the test. Otherwise, protein
degradation and denaturalization may occur in
those samples and finally lead to wrong
results.
calculation of the result
Average the duplicate readings for each
standard, control, and samples and subtract the
average zero standard optical density. Create a
standard curve on log-log graph paper, with rat
Insulin Like Growth Factor Binding Protein 2
(IGFBP2) concentration on the y-axis and
absorbance on the x-axis. Draw the best fit
straight line through the standard points and
it can be determined by regression analysis.
Using some plot software, for instance, curve
expert 1.*0, is also recommended. If samples
have been diluted, the concentration read from
the standard curve must be multiplied by the
dilution factor.
Note
The Stop Solution suggested for use with this
kit is an acid solution.Wear eye,hand,face and
clothing protection when using this material.
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