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KOD DNA polymerase
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KOD DNA polymerase
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KOD DNA polymerase

0.1 ~ 0.3 / Unit ( Negotiable )

|

1000 Unit Minimum Order

بلد:

China

نموذج رقم:

KOD DNA polymerase

سعر فوب:

0.1 ~ 0.3 / Unit ( Negotiable ) أحصل على آخر سعر

الموقع:

-

سعر الحد الأدنى للطلب:

0.1 per Unit

الحد الأدني للطلب:

1000 Unit

تفاصيل التغليف:

10000

موعد التسليم:

ONE week

القدرة على التوريد:

10000 Unit per Month

نوع الدفع:

L/C, T/T

مجموعة المنتج :

-

الاتصال الآن
عضو مجاني

الشخص الذي يمكن الاتصال به Mr. Liu

Xiangyuan Road 39#, Hangzhou, Zhejiang

الاتصال الآن

مواصفات المنتج

  • Other Names: KOD DNA polyverase
  • Type: Enzyme Preparations
  • Brand Name: INDUMY
  • colorless sotlution:colorless

الوصف

Description

DNA polymerase from Thermococcus kodakaraensis KOD is one of the most efficient thermostable DNA Polymerases with higher accuracy and elongation velocity than any other available DNA polymerase. The KOD DNA Polymerase exhibits 3’*5’ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5’*3’ exonuclease activity. This product is recombinant KOD DNA polymerase expressed in E.coli and purified to homogeneity. The enzyme generates blunt-ended PCR products suitable for cloning with any blunt-ended cloning method.

Package

             KOD DNA Polymerase (5U/μl)  Cat No. RE******3     **0 U

             *0X Buffer                                               ***0 μl

Recommend PCR Protocol

In many cases, the standard reactions described below will provide satisfactory amplification. Remember to include a negative control reaction lacking only template; inclusion of a positive control reaction using a template known to amplify with the primers may also be helpful. Concentrations of enzyme, MgCl2, template and primers can be varied to optimize the reaction..

1、For each *0μl reaction, assemble the following in a 0.5ml PCR tube on ice just prior to use.

PCR Grade Water

*4.8μl

*0×Reaction Buffer

5μl

dNTP Mixture (*0mM each, final con. 0.2mM)

1μl

Template DNA(*****0pg)

1μl

5'-primer (5pmol/μl, final concentration 0.4μM)

4μl

3'-primer (5pmol/μl, final concentration 0.4μM)

4μl

KOD DNA Polymerase (5 IU/μl)

0.2μl

total volume

*0μl

The addition of **5% DMSO can improve amplification with GC-rich or long templates and will not decrease the fidelity.

2、Mix gently and centrifuge briefly to bring reaction components to the bottom of the tube. If necessary, add mineral oil to cover the reaction, cap the tubes, and place in the thermal cycler.

3、The following are several thermal cycling program options. Note that the choice of primers affects the annealing temperature. In general use an annealing temperature 5°C below the Tm (melting temperature) of the primers as a starting point.

Phage and plasmid DNA templates

Genomic DNA

cDNA

Target

**2 kb

**4 kb

**6 kb

***0 kb

Up to 2 kb

Up to 2 kb

Denature

*0’’ *4℃

*0’’ *4℃

*0’’ *4℃

*0’’ *4℃

*5’’ *5℃

*0’’ *4℃

Anneal

2’’(Tm*5)℃

5’’(Tm*5)℃

*0’’(Tm*5)℃

*0’’(Tm*5)℃

*0’’ (Tm*5)℃

بلد: China
نموذج رقم: KOD DNA polymerase
سعر فوب: 0.1 ~ 0.3 / Unit ( Negotiable ) أحصل على آخر سعر
الموقع: -
سعر الحد الأدنى للطلب: 0.1 per Unit
الحد الأدني للطلب: 1000 Unit
تفاصيل التغليف: 10000
موعد التسليم: ONE week
القدرة على التوريد: 10000 Unit per Month
نوع الدفع: L/C, T/T
مجموعة المنتج : -

Send a direct inquiry to this supplier

إلى:

Mr. Liu < Hangzhou Biodoor Biotechnology Co,Ltd >

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