ï¬**0pcs
Disposable plastic pipette,
TEST PROCEDURE
1.Read the
instruction attentively, choose
a clean and dry place to perform the test, wash and dry your hands
before starting.
2.Take the
kit out of the fridge and wait until the temperature of the
reagents reaches the ambient temperature.
3.Calculate
how many milk sample will be tested and mark the sample tubes to
identify. The milk samples should be fully liquid without
any clots or deposition, the ideal temperature of milk sample is
between 4 to *0℃.
4.Take the
tubes required from the kit package, take out as many micro-wells
and dipsticks as the milk samples to be tested,and make
proper marks to match the milk sample.
ï¬To
open a dipsticks tube, press the safety ring down, take off the
ring and get the tube cap off with your thumb.
ï¬Recover
the cap tightly after taking out the required micro-wells and
dipsticks.
ï¬Empty
one test tube then open another one.
ï¬Keep
the unused micro-well caps tightly sealed. Do NOT tear off the
strip of 8 microwell caps.
ï¬Use
the exposed dipsticks in 1 hour.
5.Transfer
**0ul of the milk samples into the microwells, inject and reject
the milk sample for 5 times consecutively
to homogenize
the lyophilized reagent and milk sample. Start the
timer.
ï¬Dip
the end of the plastic pipette(MilkPal Mini pipette) tips into the
milk and slowly releases the sucker. When
the sucker
is back into its initial position, transfer the **0 µl of milk into
the reagents, by slowly pushing the sucker
down to
the bottom.
6.Start the timer
to incubate for 5 minutes at room temperature
(*5℃);
ï¬During
the incubation, The receptors will detect the presence of the
antibiotics in the milk sample. The reaction
will take
5 minutes.
7.Dip the dipsticks
into the micro-wells with the "Sample Pad" down side to
the bottom.
8.Start
the timer to incubate for another 5 minutes again at room
temperature.
9.Then
take out the dipsticks and place them laterally in a dry clean
place, peel off the sample pad, interpret the result.
ï¬Use
a clean nipper(or new pipette tip) to scrape gently and slowly the
sample pad.
ï¬The
peeling off of the sample pad will stop the ongoing reaction of the
receptors.
ï¬File
the used sample pad peeled off dipsticks if necessary
RESULTS
INTERPRETATION
The dipsticks are
embodied with 3 lines, Control
line, β-lactams
Line and Tetracylcines
Line, which are briefly expressed
asC,
B and
T. The test
results will be determined by the color presence of the lines. The
following diagram describes
the result
identification.
ï¬NEGATIVE: Control line, B
Line and T Line are all red, which means that, the milk sample
contains fewer antibiotics
than value
given in the table.
ï¬β-lactams
POSITIVE: Control Line is
red, B Line is invisible, which means that the milk sample contains
the beta-lactams more
than the value in the table, the tetracyclines fewer that the value
in the table.
ï¬Tetracyclines
POSITIVE: Control Line is
red, T Line is invisible; which means that the milk sample contains
the tetracyclines
more than the value in the table, the beta-lactams fewer that the
value in the table.
ï¬β-lactams and Tetracyclines
POSITIVE: Control Line is
red; neither B Line or T Line is invisible; which means that,
the milk
sample contains the the beta-lactams and tetracyclines both more
than the value in the table.
