The WatarBio DairyPal® BT is a
rapid test that detect simultaneously the presence of the
-lactams and Tetracyclines molecules
in a raw cow milk sample.
CONTENTS
ï¬*2
tubes each with 1 strip of 8 reagent microwells and 8
dipsticks.
ï¬1
Microwell holder
ï¬1
Kit insert
ï¬**0pcs
Disposable plastic pipette,
INSTRUMENT MAY NEEDED BUT NOT
PROVIDED
ï¬DairyPal® Dry
incubator(Model: Mini-B)
ï¬DairyPal® Dipstick Reader (Model:
TSR***0A, OPTIONAL)
TEST
PROCEDURE
1.Read
the instruction attentively, choose a clean and dry place to
perform the test, wash and dry your
hands before
starting.
2.Take
the kit out of the fridge and wait until the temperature of the
reagents reaches the ambient temperature.
3.Calculate
how many milk sample will be tested and mark the sample tubes
to identify. The milk samples should be
fully liquid without any clots or deposition, the ideal
temperature of milk sample is between 4 to
*0℃.
4.Take
the tubes required from the kit package, take out as many
micro-wells and dipsticks as the milk samples
to be
tested, and make proper marks to match the milk sample.
ï¬To
open a dipsticks tube, press the safety ring down, take off the
ring and get the tube cap off with
your thumb.
ï¬Recover
the cap tightly after taking out the required micro-wells and
dipsticks.
ï¬Empty
one test tube then open another one.
ï¬Keep
the unused micro-well caps tightly sealed. Do NOT tear off the
strip of 8 microwell caps.
ï¬Use
the exposed dipsticks in 1 hour.
5.Preheat the Dry Incubator(suggest to
use DairyPal Mini-B Dry
Incubator) to *5℃.
6.Place
the required numbers of the mircrowells in the dry incubator,
transfer **0ul of the milk samples into
the microwells,
inject and reject the milk sample for 5 times consecutively to
homogenize the lyophilized reagent and
milk sample.
ï¬Dip
the end of the plastic
pipette(DairyPal Mini
pipette) tips into the milk and slowly releases the
sucker.
When
the sucker is back into its initial position, transfer the
**0µl of milk into the reagents, by slowly
pushing the
sucker down to the bottom.
7.Start the timer to incubate
for 3 minutes at *5℃;
ï¬During
the incubation, The receptors will detect the presence of the
antibiotics in the milk sample. The reaction
will take 3 minutes.
8.Dip
the dipsticks into the micro-wells with the "Sample End" down
side to the bottom.
9.Start the timer to incubate
for 5 minutes at *5℃ again.
*0. Then
take out the dipsticks and place them laterally in a dry clean
place, peel off the sample pad, interpret
the result.
ï¬Use
a clean nipper(or new pipette tip) to scrape gently and slowly
the sample pad.
ï¬The
peeling off of the sample pad will stop the ongoing reaction of
the receptors.
ï¬File
the used sample pad peeled off dipsticks if necessary.
RESULTS
INTERPRETATION
The
dipsticks are embodied with 3 lines,
Control
line, -lactams Line
and Tetracyclines
Line,
which are briefly expressed as CTL, B and T.
The test results will be determined by the color presence of
the lines. The following diagram
describes the result identification.
ï¬NEGATIVE:
Line CTL is
visible, Line B and
Line T are
both stronger than or equal to Line
CTL,
which means that, the milk
sample contains fewer -lactams and Tetracyclines than value
given in the table.
ï¬-lactams
POSITIVE:
Line CTL is
visible, Line B is
weaker than Line CTL,
Line T is
stronger than Line CTL,
which means
that the milk sample contains the -lactams more than the value
in the table, the Tetracyclines fewer that
the value in the table.
ï¬Tetracyclines
POSITIVE:
Line
CTL is
visible, Line
T is
weaker than Line
CTL,
Line
B is
stronger than Line
CTL;
which means that the milk sample contains the
Tetracyclines more than the value in the table, the -lactams
fewer that
the value in the table.
ï¬FULL
POSITIVE:
Line
CTL is
visible; either Line
B or
Line
T is
weaker than Line
CTL;
which means that the milk
sample contains
the the -lactams and Tetracyclines both more than the value
in the table.
REMARKS:
Control line is used as a quality indicator, which will
always appear regardless of the line
B/T.
If Control line does
NOT appear, it indicates that the result
is
INVALID.
Users please check the kit insert again and re-do the
assay with
new test dipsticks.
SPECIFICITY
The test has no cross reaction with the
clenbuterol, ractopamine, salbutamol, melamine, trenbolone,
sulfonamides, chloramphenicol,
macrolides.
STORAGE
**8℃ in
cool dark place, do not freeze. The kit will be valid in *2
months. The lot number and expired date are
printed on
the package.
PRECAUTIONS
ï¬Do
the assay following the instruction, do
NOT touch the membrane of the
dipstick.
ï¬Seal
the bottle after taking out required dipsticks.
ï¬The dipstick is used for only once;
Do
NOT use it repeatedly.
ï¬The
positive result should be further confirmed.
