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Product: 2×Lab FastHF PCR MasterMix

Cat. No.: FH***1

Price: $*0/ml(negotiable if bulk purchase)
 

Storage temperature: **0°C for 2 years. For short-term use (within 1 month), place at 4°C

Product description:

This product contains Lab FastHF DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. When using, just add template, primers, and add water to the final concentration of Mix to 1×. FastHF DNA Polymerase comes from genetically engineered pfu, which greatly improves the amplification speed, fidelity and yield. Lab FastHF PCR Master Mix is ​​the preferred product for non-long fragment ultra-fidelity rapid amplification. This product contains red tracking dye, and can be directly loaded for electrophoresis without adding loading buffer; it can also be purified for subsequent operations such as enzyme digestion, ligation, and fluorescent sequencing. The PCR product has a blunt end and does not need to add an A head. It can be directly cloned using LabpO TOPO-Blunt blunt end series vectors (Cat. No. PB***1, PB***2).

Product features:

1. Fast amplification speed: The extension speed can reach **4kb/min, which is **8 times that of pfu.

2. High amplification yield: The general PCR product amount is *0%***0% higher than the traditional pfu yield.

3. Excellent fidelity: The fidelity is more than *4 times that of taq. Generally, a random bacterial sequencing is the correct mutation-free colony.

4. The amplification length index is consistent with pfu, and it can completely replace pfu for rapid and high-fidelity amplification, but it is not recommended for long fragments that pfu cannot amplify. Using complex genomic DNA as a template is suitable for amplifying products of no more than about 3kb, and using simple genomes, plasmids and phage DNA as templates is suitable for amplifying products of no more than about 6kb.

Notes:

1. Lab FastHF DNA Polymerase has a fast amplification speed. Simple templates such as plasmids and simple genomes can be amplified at ****0 seconds/kb; complex templates such as human genomes can be amplified at ****5 seconds/kb.

2. For templates with high GC content, the pre-denaturation and denaturation temperatures can be increased to *8°C. Lab FastHF DNA Polymerase has strong heat resistance, and *8°C has no effect on the activity of Lab FastHF DNA Polymerase.

3. If the amplification template has a high GC content or the template is complex and the amplification effect is poor, DMSO can be added to the reaction mixture to a final concentration of 1%*8%, and the optimal concentration can be found by increasing the concentration by 1%. Alternatively, betaine can be added to a final concentration of 1.**1.7 M. Touchdown PCR is used.