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2×Lab FL PCR MasterMix

CAT.No. ï¼šFL***1
Price: $*4/ml
Storage: **0°C for 2 years. For short-term use (within 1 month), it can be placed at 4°C.

Product Description:

This product contains Lab LastLong DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. When using, just add template, primers, and add water to the final concentration of Mix to 1×. This product contains red tracking dye, and can be directly loaded for electrophoresis without adding loading buffer; it can also be purified for subsequent operations such as enzyme digestion, ligation, and fluorescent sequencing. The PCR product is 3 with an A head at the end. After purification, it can be directly cloned using LABLEAD LabpO TOPO TA series vectors (Cat. No.: PA***1, PA***2).

Product Features:

1. Super fast amplification speed: The extension speed can reach ***0sec/kb, and PCR can be completed in as short as half an hour.

2. Outstanding long fragment amplification ability: It can amplify long fragments up to *0kb (including complex templates such as the human genome).

3. Good fidelity: The fidelity is more than 3 times that of taq.

4. Strong amplification ability: high yield, good effect for some complex templates with special structures and templates with high GC content.
Notes:

1. This product has a very fast amplification speed. Simple templates such as plasmids and simple genomes can try 5s/kb extension speed and reduce the number of cycles to further shorten the PCR time; long fragments (greater than or equal to 3kb) or complex templates with low yield should use ****0s/kb extension speed or more cycles.

2. When the Lab FL yield is low, you can try to use a longer extension time (such as *0s/kb) and a longer number of cycles (such as *5) to increase the product yield.

3. For templates with high GC content, the pre-denaturation and denaturation temperature of short fragments can be increased to *8 oC and/or the denaturation time can be appropriately extended. In order to avoid DNA damage, the denaturation temperature of long fragments should not be increased, and the pre-denaturation time should be appropriately extended to 5min., and the denaturation time can be extended by ***0s.

4. If the amplification template has a high GC content or the template is complex and the amplification effect is poor, DMSO can be added to the reaction mixture to a final concentration of 1%*8%, and the optimal concentration can be found by increasing the gradient by 1%. Alternatively, add betaine to a final concentration of 1.**1.7 M. Use touchdown PCR