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Porcine Toxoplasmosis IgG Antibody ELISA test kit
Catalog No. LSY*****1
1. Usage
Porcine Toxoplasmosis IgG antibody test kit is used for the detectionof the Toxoplasmosis antibodies in porcine serum.
 
2. Principle
The test kit is enzyme immunoassay for the detection toxoplasmosis antibodies in porcine serum. Toxoplasmosis antigens are coated on **-well plate, add sample into wellsand then antibodies bound specifically by antigens, the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation to specifically bind with the compound of antibodies and antigens on the microplate. Theunbound enzyme conjugation will be removed by washing. Add the TMB substrate in the well, react to a blue product. At last, end the reaction by adding stop solution.
 
3.Components
1  Antigen coated microplate.......................................2 pieces (*6 wells each)
2  TOX-IgG Negative control serum (green lid)................................1.5 mL/tube
TOX-IgG Positive control serum (red lid).....................................1.5 mL/tube
3  Enzyme conjugate (yellow lid).....................................1 bottle (*2 mL/bottle)
4  *0×concentrated washingsolution (white lid)...............1 bottle (*0 mL/bottle)
5  Substrate A (purple lid)/B (yellow lid)...................1 bottle each (*2 mL/bottle)
6  Stop solution (blue lid)...............................................1 bottle (*2 mL/bottle)
7  Sample diluentsolution (transparent lid)......................1 bottle (*5 mL/bottle)
8  Adhesive Foil.................................................................................6 pieces
9  Instruction.......................................................................................1 piece
(Note: Never mix up reagents from test kits in different batch number)
 
4. Material Required not Provided
 

1. Microplate Reader (single-wave length: **0nm or double-wave length: **0/**0 nm).
2. Microplate Washer.
3. Micropipettes, adjustable.
4. Constant temperature box or water bath box.
5. Oscillator.