Description
Product name :2×Taq PCR Mix (including dye)
Cat. No. T***1
Product number: T***1
Storage conditions: **0℃
Product description
The concentration of Taq PCR Mix (containing dye) is 2×. It is easy
and quick to use and can reduce contamination during PCR operation.
When using, just take an appropriate amount of 2×Taq PCR Mix
(containing dye), add template and primer, and add ddH2O to make up
the volume, so that the concentration of the reaction system is 1×,
and then you can carry out PCR reaction. The Taq in this Mix is
​​the mutant Taq-AS (Advanced Strong) DNA Polymerase obtained
by screening. It can efficiently amplify DNA fragments ≤5Kb and has
good inhibitor tolerance. Its amplification speed is about 3 times
that of ordinary Taq DNA polymerase. Therefore, it can greatly
reduce the time required for the PCR extension process, so as to
shorten the entire PCR reaction. Different from the fast Taq
polymerase based on the fusion protein principle, the use of Taq-AS
is closer to WT-Taq, and it is not easy to have electrophoretic
band diffusion, dragging or fragment size changes. This PCR Mix
contains two dyes. The PCR product can be directly spotted for
electrophoresis without adding Loading Buffer, and two indicator
bands, purple and yellow, will appear during the electrophoresis.
The dye does not affect the PCR amplification efficiency, but for
experiments that require optical analysis of the PCR product such
as absorbance and fluorescence, it is recommended to purify the PCR
product before analysis. The PCR product has an A base at the 3\'
end and can be directly used for T/A cloning after
purification.
Quality Control
Endonuclease Residual Detection
The enzyme solution was incubated with supercoiled plasmid DNA at
*7℃ for 4h, and no change in the plasmid was detected by DNA
electrophoresis.
Exonuclease Residual Detection
The enzyme solution was incubated with double-stranded DNA
substrate at *7℃ for *6h, and no change in the double-stranded
DNA substrate was detected by DNA electrophoresis.
Stability Test
No obvious activity change after storage at room temperature for
one week.
Functional detection
Using Arabidopsis genomic DNA and human genomic DNA as templates, a
5kb fragment was amplified, and the target band was visible after
*0 cycles of agarose gel electrophoresis.
